Chemistry of bleomycin. XVI. Epi-bleomycin.

Chemistry of bleomycin. XIX Revised structures of bleomycin and phleomycin.

Chemistry of bleomycin. XVIII. carbon-13 NMR studies.

During chemical studies of bleomycin, many fragments and derivatives have been isolated and characterized. The 13C-NMR spectra of these compounds were taken and analyzed for structural information, and the complete assignment of the spectra was achieved. The 13C-chemical shift map thus obtained contains information about the structure and conformation and will be useful for studies on the chemi...

Chemistry of bleomycin. XX. The X-ray structure determination of P-3A Cu(II)-complex a biosynthetic intermediate of bleomycin.

Sir: During a biosynthetic study of bleomycin (BLM), several peptides, structurally related to BLM, were isolated from the culture broth of Streplomyces verticillus. One of them, designated as P-3A, was crystallized from water (NAKATANI et al., unpublished) as an equimolar Cu(II)-complex. Acid hydrolysis of metal-free P-3A gave ~-aminoalanine (amine component V" of BLM), 2-(I-amino-2-carboxyeth...

Radioimmunoassay of bleomycin.

A radioimmunoassay for bleomycin has been produced using 125l-labeled bleomycin and antisera raised in rabbits against a carbodiimide-catalyzed bleomycin-bovine serum albumin conjugate. 125l-Labeled bleomycin was synthesized by direct iodination of the drug using the chloramine-T technique. The standard curve of the assay was linear on a logit-log plot and the lower limit of sensitivity was 250...

Pulmonary toxicity of bleomycin.

Diffuse pulmonary fibrosis is associated with bleomycin administration to humans. The sequential reactions of lung cells to this drug have now been investigated in mice following injection of 20 mg/kg bleomycin twice per week for 4 to 8 weeks. Cytoplasmic and subendothelial edema was first observed in large vessels and by 4 weeks involved the capillaries. The reaction in many animals did not pr...